In this research. Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein. The objective of this research was to figure out the optimum time of mpt64 gene expression through real-time monitoring of MPT64 protein production and distribution in host compartments. ... https://www.bekindtopets.com/super-value-Blue-Lotion-Topical-Antiseptic-16-oz-flash-save/